Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist

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Diagnostic value of direct immunofluorescence of mucosal and skin biopsy specimens on formalin-fixed paraffin-embedded biopsy specimens compared to specimens placed in normal saline as the gold standard method in the diagnosis of a group of autoimmun

Background and Aim: Direct immunofluorescence (DIF) is represented as a gold standard method in diagnosis of autoimmune blistering dermatoses. Normal saline, liquid nitrogen and michel’ solution are a widely accepted media for preserving biopsy samples of skin or mucosa before DIF examination. Occasionally clinicians put the biopsy specimen taken for DIF in formalin 10%, occasionally clinicians...

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Direct immunofluorescence of skin using formalin-fixed paraffin-embedded sections.

The technique of direct immunofluorescence has been applied to skin biopsy specimens fixed in formalin and embedded in paraffin wax. The results have been compared with those obtained by using snap-frozen biopsy specimens from the same patients. Trypsinisation of the dewaxed material allowed subsequent detection of immunoglobulins, complement, and fibrinogen. When compared to the fluorescence i...

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ژورنال

عنوان ژورنال: Indian Journal of Dermatology, Venereology, and Leprology

سال: 2010

ISSN: 0378-6323

DOI: 10.4103/0378-6323.60561